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ARTICLES详细介绍
产品特点:
1、组织不必研磨,不用过夜消化,直接剪入试剂板,1.5小时左右提取DNA,DNA长度50kb~200kb,260/280:1.7~2.0,260/230≥1.5,特别适合于容易降解的动物肝脏组织,胰脏组织,海洋水产样本、昆虫样品。
2、全血DNA提取得率:人5-12ug/200ul,羊4-10ug/200ul,猪牛5-12ug/200ul,禽类鸡鸭50-80ug/20ul,DNA长度50kb~200kb,260/280:1.7~2.0,260/230≥ 1.5。
3、血清、血浆、细胞、细菌、体液、拭子等均可直接提取,DNA完整性高于离心柱试剂盒,得率纯度和离心柱试剂盒相当。
4、超高灵敏度病毒DNA、RNA提取技术,使你轻松提取血清中微量的病毒DNA、RNA及微量游离核酸。
5、通量大,一次提取32个样本,8小时提取300个左右样本,仪器可以申请投放!
iAUTOMAG技术参数
★ 处理体积:20uL-1000uL
★ 样品通量:1-32
★ 磁珠回收效率:>95%
★ 板类型:96孔板
★ 提纯灵敏度:102拷贝样品的阳性检出率 > 95%
★ 提纯孔间差:CV < 3%
★ 磁棒:32
★ 磁棒表面磁强:大于4000Gs
★ 加热:可选配加热模块,实现裂解加热(室温至 + 80℃)与洗脱加热(室温至 + 80℃)
★ 振荡混合:多模式多档可调
★ 磁珠大小:>100nm
★ 操作界面:全中文大屏幕彩色液晶 + 触控操作
★ 内部程序:内建15组模式程序
★ 仪器扩展接口:USB
★ 程序管理:新建、编辑、删除,模式程序
★ 杀菌消毒:紫外灯
★ 操作时间:30-60分钟/次
★ 网络通讯:可扩展以太网通讯
实例:
1. 从组织中提取基因组DNA (牛肌肉组织)
凝胶电泳分析
Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 20mg of bovine tissues and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have very similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).
2. 在细胞中提取基因组DNA (Hela细胞系)
琼脂糖凝胶电泳分析
Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 1X106 cells of cultured HeLa cell and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have very similar yields. Purity was also tested and was consistently between 1.8 and 2.0 (not shown).
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